Principal Investigator: Amadou A. Sall, Institut Pasteur de Dakar
Real‐time Reverse Transcription Polymerase Chain Reaction(RT-PCR) was used as the standard method for Ebola virus (EBOV) molecular diagnosis during the 2014 outbreak in West Africa but has some limitations in terms of cost, equipment, and turnaround time. In contrast, isothermal recombinase polymerase amplification (RPA) is six times faster than RT- PCR while yielding the same analytical sensitivity and specificity. In addition, the RPA assay uses smaller equipment and reagents which are cold chain independent, making rapid on-site testing feasible and affordable. The project investigated the use of RPA for EBOV point-of-care detection in Guinea treatment centres.
An existing Ebola virus Recombinase polymerase amplification (RPA) assay was adapted to the outbreak strain and mobilized into a suitcase laboratory combined with a mobile glovebox for biosafety sample extraction. Specifically, so called primer–in pellets were developed in collaboration with company Twist DX allowing for cooling chain independent and simple use of reagents. The RPA assay was deployed in Conakry as well as during campaigns organized by the coordination committee in the Forecariah, Coyah, Dubreka and Boke prefectures. During these campaigns, 2,509 samples were tested and the results were available within 30 minutes upon sample receipt.
International Team Begins Field Trials for Solar-Powered 'Suitcase Lab,' Ebola AssayView
The project is focussing on developing suitcase labs which can speed up testing. The portable labs are testing after death and producing results in between six to ten minutes which means that burials can take place faster and within culturally sensitive time frames.View
In August 2014, the Ebola Outbreak in West Africa was declared an International Health Emergency by WHO and within a couple of weeks ELRHA launched a rapid-response call for research to combat the crisis. The UK Department for International Development (DFID), the Wellcome Trust and ELRHA opened a special funding window through the Research for Health in Humanitarian Crises (R2HC) programme.
The aim of this emergency call was both to produce robust research findings that could contribute to the effectiveness of the response to the current outbreak and help to draw lessons for future outbreaks of Ebola and other communicable diseases. The projects funded will strengthen the evidence base for the Ebola response in topics ranging from diagnostics to anthropology, surveillance and disease control.
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